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. 2016 Feb 1;11(2):e0148217. doi: 10.1371/journal.pone.0148217

Fig 4. Induction of apoptosis by Tax1 in growing cells.

Fig 4

(A) Growing or resting Kit 225 cells were infected with Ad-Tax1 or Ad-Tax2B, and cultured for 72 h. DNA fragmentation was detected by the TUNEL assay using a flow cytometer. The thick line and gray area indicate Ad-Tax1- or Ad-Tax2B-treated cells and Ad-Con-treated cells, respectively. (B) Percentage averages number of the cells undergoing apoptosis was calculated from three independent experiments. Values are shown as the means ± SE. *, p < 0.05. (C) DNA fragmentation and Tax1 expression were measured using a flow cytometer. Tax1 was detected with biotin-labeled anti-Tax1 antibody (Lt-4) and phycoerythrin-conjugated streptavidin, and DNA fragmentation was measured by TUNEL assay.