Skip to main content
. 2016 Feb 1;11(2):e0148217. doi: 10.1371/journal.pone.0148217

Fig 9. Induction of chemokines and cytokines by Tax1-mediated RelA activation.

Fig 9

(A) Growing or resting Kit 225 cells were infected with Ad-Tax1 or Ad-Tax2B, and cultured for 72 h. The cytoplasmic and nuclear extracts were subjected to western blotting with antibodies for RelA, Tax1, Tax2, β-Tubulin and nucleolin. (B) The reporter plasmid carrying the NF-κB-binding sites was transfected into Kit 225 cells along with the Tax1 and Tax2B expression plasmids (pMT-2Tax and pHβAP-r-1-neoTax2B, respectively). After 48 h of culture with or without IL-2, the cells were harvested for luciferase assay, which was further normalized against protein content. Values are shown as the means ± SE. (C and D) Growing or resting Kit 225 cells were infected with Ad-Tax1 or Ad-Tax2B, and cultured for 72 h (C). siRNA-treated growing and resting Kit 225 cells were infected with Ad-Con or Ad-Tax1, and cultured for 48 h (D). Gene expression was monitored by RT-PCR. 18 S rRNA was used as an internal control.