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. Author manuscript; available in PMC: 2016 Oct 6.
Published in final edited form as: Anal Chem. 2015 Sep 10;87(19):10096–10102. doi: 10.1021/acs.analchem.5b02766

Figure 1.

Figure 1

Selectivity and sensitivity screening of a G7 PAMAM dendrimer-immobilized platform with antibodies against surface markers using in vitro murine lung cancer cell lines, ED-1 and ED1-SC. a) A schematic illustration of this study investigating polymer chemistry, sensitivity, and specificity. b) Enhanced binding between anti-EGFR and cancer cells through dendrimer-mediated multivalent binding. c) Antibody screening for capturing of mouse lung cancer cells. All antibodies were functionalized on dendrimer-immobilized surfaces. Anti-EGFR antibody showed the highest binding with the both lung cancer cells, but not with HL-60, a leukocyte model. d) High recovery of the both cells from cell mixtures (in a range of 40-10,000 ED-1 or ED1-SC cells mixed with 1 × 107 HL-60 cells were used) using anti-EGFR. (n=3, All error bars: standard error (S.E.)).