Skip to main content
. Author manuscript; available in PMC: 2016 Feb 1.
Published in final edited form as: ACS Appl Mater Interfaces. 2015 Feb 24;7(8):5017–5028. doi: 10.1021/acsami.5b00655

Figure 3.

Figure 3

Cell uptake of nanoparticle-encapsulated PI (nPI) and negligible cytotoxicity of empty GNPs. a) Typical micrographs of primary human adipose-derived stem cells (hADSCs) at 24 h after incubating with free PI, a simple mixture of free PI and GNPs, and nPI together with that after 1 h incubation with nPI. The PI concentration was 0.08 mM for all the conditions. b) Typical flow cytometry peaks and quantitative fluorescence intensity of GNPs in hADSCs incubated with nPI for various times showing quick cell uptake of the GNPs in 1 h and decrease in fluorescence intensity of the GNPs thereafter possibly due to degradation of the GNPs inside the cells. c) Typical flow cytometry peaks and quantitative intensity of PI in the hADSCs incubated with nPI for various times at 37 °C showing significantly higher intracellular PI intensity at 24 h than 1 or 6 h. d) Proliferation of the hADSCs after incubating with empty GNPs at various concentrations for 1, 2 and 3 days. *: p < 0.05; **: p < 0.01.