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. Author manuscript; available in PMC: 2016 May 12.
Published in final edited form as: Nature. 2015 Oct 19;527(7577):259–263. doi: 10.1038/nature15391

Extended Data Figure 2. Functional analysis of SWEET activities by a yeast growth assay.

Extended Data Figure 2

When tested for complementation of the growth defect of the EBY4000 mutant strain, OsSWEET1a and OsSWEET1b showed limited growth on glucose, while OsSWEET2a and OsSWEET2b did not show growth. When tested on the toxic glucose analogue 2-deoxyglucose, only OsSWEET1a and OsSWEET1b failed to grow, suggesting that they may be glucose transporters. In contrast, OsSWEET2a and OsSWEET2b were able to grow in the presence of the 2-deoxyglucose, possibly because they are localized to the vacuole membrane and are not able to mediate uptake of the sugar analogue.