Fig 3. Translation of chloroplast proteins is significantly impaired in aladin-1.

(A-B) Protein extracts from 7-day-old wild-type (WT) and aladin-1 seedlings were subjected to SDS-PAGE followed by either Coomassie Brilliant Blue staining (A) or immunoblotting (B) with anti-RuBisCO large subunit (RBCL), RuBisCO small subunit (RBCS), light-harvesting chlorophyll a/b binding protein (LHCP), photosystem II reaction center protein A (PSBA), plastoglobulin 35 kDa (PGL35), or binding protein (BiP) antibodies. Molecular masses are indicated on Fig 3A (kDa). Technical duplicate was done: two independent extractions of protein (A) and mRNA (B) were subjected to two lanes on each panel. (C) RT-PCR analysis of RBCL, RBCS, PSBA, and ACT2 transcription in WT and aladin-1. Technical duplicate was done: two independent extractions of mRNA were subjected to two lanes on each panel.