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. 2016 Feb 1;11(2):e0147867. doi: 10.1371/journal.pone.0147867

Fig 8. Comparison of SALSA co-localization with fibronectin and C1q.

Fig 8

Placental sections of healthy term placentas were stained with a polyclonal anti-fibronectin antibody (A, green) and a polyclonal anti-C1q antibody (B, green). SALSA was stained using monoclonal anti-SALSA antibody (Hyb 213–06, panels C and D, red). Staining was visualized by immunofluorescence microscopy (200x magnifications). Superimpositions of the stainings are shown in (E) and (F). SALSA and fibronectin are partially found in the same structures in the placenta (yellow, white arrows in panel E). SALSA is located mainly in the inner mass of the fibrinoid structures, while fibronectin is mostly located at the edges of the structures. C1q and SALSA are also found in the same placental structures. However, no direct co-localization was observed. Instead, C1q appears to coat the SALSA-positive fibrinoids (white arrows in panel F). 200× magnifications.