Fig 1. Clinical course of neonatal ddY mice after intracerebral inoculation with SAFV-3.

Within 24 h of birth, neonatal ddY mice were inoculated intracerebrally with 10⁴ CCID₅₀ (cell culture infectious dose) of the aseptic meningitis (AM) or upper respiratory (UR) strains of SAFV-3. Animals were observed for clinical manifestations, and body weight was measured daily for 15 days and on Day 21 (n = 3–4 mice per group). Two of the four AM-inoculated mice showed mild neurological signs, such as rolling and ataxia, on Days 7 and 8 post-inoculation (p.i.) (arrows), but the animals rapidly recovered. UR-inoculated mice showed significantly less weight gain than mock-infected control mice. All animals survived the inoculation. (*P < 0.05 and ***P < 0.001; one-way ANOVA). The data in the figure are representative of two experiments with similar results.