Skip to main content
. 2016 Feb 1;11(2):e0148294. doi: 10.1371/journal.pone.0148294

Fig 3. AP-dependent autophosphorylation reactions of mutated ArcB, EvgS, and BarA proteins.

Fig 3

The G2-box-mutated proteins, ArcB G2* (A), EvgS G2* (B), or BarA G2* (C), the proteins ArcB H292A (A), EvgS H721A (B), or BarA H302A (C), which were His-mutated in the HK domain; ArcB D576A (A), EvgS D1009A (B), and BarA D718A (C), which were Asp-mutated in the receiver domain; and ArcB H717A (A), EvgS H1137A (B), and BarA H861A (C), which were His-mutated in the HPt domain, were autophosphorylated in the presence of AP, and then analyzed by Phos-tag SDS-PAGE [8% (w/v) polyacrylamide and 20 μM Mn2+–Phos-tag]. Each lane contained 2.0 μg proteins.