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(A) The reactions of ArcB autophosphorylation and ArcB/ArcC phosphorelay were analyzed by Phos-tag SDS-PAGE [8% (w/v) polyacrylamide and 20 μM Mn2+–Phos-tag]. Each lane contained 2.0 μg of ArcB or ArcC. (B and C) Schemes of modes of autophosphorylation and phosphoryl-transfer reactions, based on the Phos-tag SDS-PAGE gel images shown in A.
