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. 2016 Feb 1;11(2):e0148202. doi: 10.1371/journal.pone.0148202

Fig 1. In situ hybridization showing the expression of Hapln1a-ECM components on 5 dpa regenerating fins.

Fig 1

(A) Whole mount in situ hybridization of components of the Hapln1a-ECM. The genes has1, has2, and acanb are expressed during fin regeneration. The amputation plane is indicated by a black line in all the panels. Scale bar represents 100μm. (B) In situ hybridization on a WT 5 dpa cryo-section reveals compartmental expression of has1, has2, and acanb. Blastema (b), mesenchyme (m), and skeletal precursor cells (*). The thick arrow identifies the basal layer of the epidermis, which underlies the epidermis (e). The thin arrow identifies lepidotrichia and the arrowhead identifies the actinotrichia. Scale bar represents 50μm.