Fig 4. Double knockdown experiments suggest that HA produced by has1 and has2 may be functionally distinct.

Double knockdown of has1 and has2 was performed by injecting/electroporating combined 1 mM stocks of each ATG-blocking MO (or of each MM MO), resulting in 0.5 mM concentrations of the each MO. This was compared to single knockdowns of 0.5 mM concentrations for each targeting or MM MO. Bar graphs of percent similarity show that the double knockdown does not result in synergistic defects for regenerate length (A), segment length (B), or cell proliferation (C). Students t-test was performed (p<0.05) to determine significance, and the error bars indicate standard error of the mean. None of the double knockdowns were statistically different from the knockdown of has2 alone (i.e. which is the only single knockdown to show phenotypic effects).