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. 2016 Jan 27;11:399–411. doi: 10.2147/IJN.S84927

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© 2016 Zhu et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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The results of HepG2 cells transfected with anti-miR-221 using PCPH NPs.

Notes: (A) Relative expression change of miR-221 level in HepG2 cells. (B) Western blot analysis of p27^Kip1 in the HepG2 cells after treatment with different formulations. (C) Cell cycle profiles of HepG2 cells after treatment with different formations; (D) cell proliferation of HepG2 cells after treatment with different formations (images obtained 3 days after treatment); (a) untreated group; (b) pure PCPH NPs; (c) PCPH/mismatched AMO-transfected HepG2 cells; (d) PCPH/anti-miR-221-transfected HepG2 cells. Magnification of the images is 200×.

Abbreviations: PCPH, polylactic-co-glycolic acid/cetylated polyethyleneimine/hyaluronic acid; NPs, nanoparticles; AMO, anti-microRNA oligonucleotide; miR, microRNA; NC, negative control.