From data of
ex vivo cultures of resting CD4
+ T cells isolated from HIV
+ patients (Table
3), the extracellular HIV‐1 genomic RNA levels for each LRA treatment are represented from all patient cell cultures (A), from patient cell cultures presenting no viral reactivation in mock condition (C), and from patient cell cultures exhibiting reactivation in mock condition (B). One night after cell purification, cells were mock‐treated or treated with 5‐AzadC. Three days post‐treatment, 1/3 of medium was replaced and HDACIs were added in the cultures. Six days after 5‐AzadC treatment, the concentration of viral RNA in culture supernatants was determined (in copies/ml). The results were reported as the actual HIV RNA copy numbers/ml or as an estimated value calculated as 50% of the smallest value when HIV RNA was not detected in order to assign a log value. Means are represented. Nonparametric one‐way ANOVA for independent samples (Kruskal–Wallis) followed by paired comparisons between each treated condition and the mock‐treated condition (Mann–Whitney test) are performed.
