Figure 9. 5‐AzadC + panobinostat and 5‐AzadC + romidepsin treatments do not induce global T‐cell activation.

• A–H
  One night after cell purification, CD8⁺‐depleted PBMCs (E–H) or HLA DR ⁻ CD69⁻ CD25⁻ CD4⁺ T cells (A–D) from 4 HIV‐negative donors were mock‐treated or treated with 5‐AzadC. Three days post‐treatment, 1/3 of medium was replaced and HDACIs were added to the cultures. The activation status of CD4⁺ T‐cell subset was assessed 6 days after 5‐AzadC treatment by flow cytometry analysis of cellular activation markers relative to mock treatment before 5‐AzadC stimulation corresponding to day 0. Means are represented. Nonparametric one‐way ANOVA for independent samples (Kruskal–Wallis) followed by paired comparisons between each treated condition and the mock‐treated condition (Mann–Whitney test) are performed.