Figure 10. 5‐AzadC + panobinostat and 5‐AzadC + romidepsin treatments induce a significant decrease in the cell surface CD4 receptor expression.

One night after cell purification, HLA DR ⁻ CD69⁻ CD25⁻ CD4⁺ T cells from 4 HIV‐negative donors were mock‐treated or treated with 5‐AzadC. Three days post‐treatment, 1/3 of medium was replaced and HDACIs were added to the cultures. The median fluorescence intensity of CD4 receptor of viable CD4⁺ T‐cell subset was assessed 6 days after 5‐AzadC treatment by flow cytometry analysis relative to mock treatment before 5‐AzadC stimulation corresponding to day 0. Means are represented. Nonparametric one‐way ANOVA for independent samples (Kruskal–Wallis) followed by paired comparisons between each treated condition and the mock‐treated condition (Mann–Whitney test) are performed.