Fig 1.

Development of dermatitis and atopy in filaggrin-deficient mice. A, Macroscopic clinical scoring of Flg^ft/ft versus WT mice. Data are from 25 to 30 mice per strain (scored longitudinally). Statistical significance was determined with 2-way ANOVA. B, Gross phenotype of a representative Flg^ft/ft neonate in comparison with a WT littermate. C, Gross phenotype of Flg^ft/ft and WT mice (age matched at 12 weeks). D, Representative image of the eczematous inflammation that develops in the eyelid skin of Flg^ft/ft mice at 12 weeks. E, Representative hematoxylin and eosin–stained biopsy specimens of eyelid skin from 12-week-old Flg^ft/ft and WT mice. Scale bar = 200 μm. F and G, Epidermal acanthosis scoring (Fig 1, F) and dermal eosinophil, neutrophil, and lymphocyte counts per high-power field (HPF; Fig 1, G) in lesional skin. Data are from 6 to 10 mice per strain. H, Total serum IgE levels from adult mutant and 12-week-old age-matched WT mice. I, Transepidermal water loss (TEWL) at 12 weeks. Data are from 23 to 38 mice per strain. *P < .05, **P < .01, and ****P < .0001.