Fig 4.
Spontaneous lung inflammation in filaggrin-deficient mice. A and B, Measurement of AHR as assessed by Cdyn (Fig 4, A) and RL (Fig 4, B) in response to increasing doses of methacholine. Data are representative of 3 experiments (n = 6-8 mice per group). Statistical significance was determined with 2-way ANOVA. C, Differential cell counts from bronchoalveolar lavage fluid. Data are representative of 3 experiments (n = 6-8 mice per group). Statistical significance was determined with the Student t test. D and E, Representative hematoxylin and eosin–stained (Fig 4, D) and Masson trichrome–stained (Fig 4, E) lung tissue. Scale bars = 200 μm. F, Cytokine quantification in the lung expressed as nanograms of cytokine per milligram of protein. Data are from 16 to 20 mice per strain. Statistical significance was determined with the Student t test. *P < .05, **P < .01, and ***P < .001. NS, Nonsignificant.