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Changes in muscle function after 5, 10 and 25 weeks of high-fat diet. Muscle function tests include the (a) hang wire, (b) hang mesh and (c) grip strength test. To account for potential effects of body weight, hang wire and hang mesh results are multiplied by body weight. At each time-point, Mann–Whitney U-tests were performed to examine differences between chow and high-fat fed mice. Data are presented as mean±s.e.m. **P<0.001; Unfilled bars, chow; filled bars, high-fat diet.
