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. 2016 Jan 22;5(1):e59. doi: 10.1038/cti.2015.41

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Copyright © 2016 Australasian Society for Immunology Inc.

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RT-PCR for ITPKB mRNA. RT-PCR was performed on activated PBMCs (anti-CD3 for 6 h) from the patient and two healthy controls using specific primers and ACTB as a housekeeping gene. Top panel (a) shows a gel and the bottom panel (b) show quantitation of gels by densitometer. A significant decreased in ITPKB mRNA was observed.