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In the closed form of the active-site loop of pyranose oxidase from T. multicolor (TmPOx, PDB code 1TT0; [3]), which is thought to be relevant for the oxidative half-reaction of POx [3], Phe454 is positioned in the direct vicinity of the isoalloxazine ring and the C4a/N5 locus, at which oxygen is reduced. The figure was generated using PyMOL (http://www.pymol.org/).
