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Steady-state kinetic constants were determined for wild-type TmPOx and its variants with D-glucose (0.1–20 mM) as electron donor, and O2 (air) under saturation as electron acceptor.
| kcat (s-1) | Km (mM) | kcat/Km (mM-1s-1) | |
|---|---|---|---|
| wt POx1 | 49.0 ± 1.7 | 0.851 ± 0.140 | 57.6 |
| F454A | 0.479 ± 0.031 | 0.712 ± 0.011 | 1.47 |
| F454Y | 16.3 ± 0.3 | 0.948 ± 0.08 | 17.2 |
1 Recombinant POx from T. multicolor overexpressed in E. coli carrying a His6-tag
