Figure 8. Whole-mount in situ hybridizations to pax5 and pax2a.
(a–l) Lateral views of control (ConMO) and HDAC1 morphant otic vesicles at 26 hpf, 32 hpf, and 48 hpf. Black brackets indicate the localization of pax5-expressing (a–c) and pax2a-expressing (g–i) cells as appropriate. Embryos injected with HDAC1 morpholino show reductions in the levels of pax5 (d–f) and pax2a (j–l) expression. The otic vesicles are outlined by dashed lines. All images show lateral views with the anterior to the left and the dorsal side up.