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. 2016 Feb 2;7:55. doi: 10.3389/fmicb.2016.00055

Table 4.

Analyses of ICESa2603-family fragments in part of macrolide and tetracycline resistant S. suis by PCR tiling assay.

Fragmentsa attL 1 2 3b 4 5 6 7 8 attR
HB1004 + + - - + + + + + +
HB1006 - - - - + + + + + +
HB1011c + + + - + + + +b + +
HB1012 + + - - + + + + + +
HB1013 - - - - + + + - - -
NJ4 + - - - + + + + - -
JS07002 + + - - + - + + + +
JS07015 + + - - + + + + + +
SC05017 - - - - + + + + + -
SS2-XY + - - - + + + + + +
YY060816 - - - - + + + + + -
a

The PCR tiling assay and fragments can be seen in Figure 1E.

b

Failure of amplifying this fragment, probably due to too long additional DNA segments inserted in the 4 regions (I-1 to I-4)

c

The fragment 7 in HB1011 was ~2 kb larger than ICESa2603 core region.