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. 2016 Feb 2;10:6. doi: 10.3389/fncel.2016.00006

Figure 1.

Figure 1

Expression of NOS1AP-L or NOS1AP-S in COS-7 cells decreases F-actin. (A) Schematic of NOS1AP-Long and NOS1AP-Short protein domains. The long isoform of NOS1AP produces a protein with a PTB domain, CPE-binding region, and a PDZ-binding motif (NOS1AP-L). The short isoform of NOS1AP produces a protein with only the PDZ-binding motif (NOS1AP-S). The first 17 amino acids of NOS1AP-S are not present in NOS1AP-L. (B) Upper, extracts from cultures of transfected COS-7 cells expressing GFP (control), NOS1AP-L, or NOS1AP-S were resolved by SDS-PAGE and analyzed by Western blotting using antibodies that recognize actin or GAPDH. Representative blot is shown. Lower, densitometry analysis of multiple blots represented in upper. Error bars indicate ± s.e.m. n = 6 for all conditions. a.u., arbitrary units. (C) Upper, extracts from cultures of transfected HEK293T cells expressing GFP (control), NOS1AP-L, or NOS1AP-S were resolved by SDS-PAGE and analyzed by Western blotting using antibodies that recognize actin or GAPDH. Representative blot is shown. Lower, densitometry analysis of multiple blots represented in upper. Error bars indicate ± s.e.m. n = 5 for all conditions. a.u., arbitrary units.