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. 2016 Feb 2;7:7. doi: 10.3389/fphar.2016.00007

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Copyright © 2016 Mai, Yap, Kho, Ismail, Yusoff, Shaari, Chin and Lim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytotoxicity of Clinacanthus nutans extracts on cells. Dose response curve of LP, LN, SP, and SN on cell viability of human embryonic kidney cells, HEK-Blue^TMhTLR-4 (A) and murine macrophages, RAW264.7 (B). All values were not significant difference when compared to 0.1% DMSO (one-way ANOVA post hoc Dunnett’s t-test). Each value represents means ± SD from three independent experiments. (C) No significant morphological changes in HEK-Blue^TMhTLR-4 and RAW264.7 cells after 72 hours treatment with 100 μg/mL of extracts or 0.1% DMSO.