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. 2016 Feb 1;11(2):e0148282. doi: 10.1371/journal.pone.0148282

Fig 1. Transcription reaction.

Fig 1

Transcription with 5S rRNA DNA templates (oligomer O1 and O2, S1 Table) using four natural NTPs (lanes 1, 6, 11, 16) or 5’NH ATP and 3 natural NTPs (lanes 2, 7, 12, 17) or 5’NH CTP and 3 natural NTPs (lanes 3, 8, 13, 18) or 5’NH GTP and 3 natural NTPs (lanes 4, 9, 14, 19) or 5’NH UTP and 3 natural NTPs (lanes 5, 10, 15, 20). Lanes 1–5 –RNAP T7. Lanes 6–10 –RNAP T7 Y639F. Lanes 11–15 –RNAP T3. Lanes 16–20 –RNAP Sp6. The gel is a representative of three replicate experiments.