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. 2016 Jan 13;6:661–667. doi: 10.1016/j.dib.2016.01.004

Fig. 2.

Fig. 2

The expression and purification of rL-CRBGP. The rL-CRBGP was separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and stained with Coomassie brilliant blue R-250. Lane M, low molecular weight protein marker; lane 1, crude lysate of Rosetta blue cells before IPTG induction (28 μg); lane 2, crude lysate of Rosetta blue cells after induction with 1 mM IPTG for 36 h (30 μg); lane 3, precipitation from the induced Rosetta blue cells after sonication on ice for 1 h (33 μg); lane 4, supernatant from the inducted Rosetta blue cells after sonication on ice for 1 h (28 μg); lane 5, the purified rL-CRBGP (7.5 μg).