Fig. 5.

rL-CRBGP inhibited basic fibroblast growth factor (bFGF)-induced angiogenesis in CAM assays. Similar to the anti-angiogenic assay of native lamprey CRBGP in CAM models, PBS (control) and rL-CRBGP (2, 10, 20 and 40 μM) were also added onto the methylcellulose disc implanted into the bFGF-induced chick embryos at 37 ˚C for 24 h, respectively. When the methylcellulose disc was removed, the chick embryos were captured by digital camera (Nikon, Japan).