Skip to main content
. 2016 Feb 1;11(2):e0148439. doi: 10.1371/journal.pone.0148439

Fig 7. OGR1-KO macrophages have a greater ability than WT macrophages to inhibit the proliferation and cytokine production by CD4+ T cells.

Fig 7

CFSE-labeled WT CD4+ T cells were plated onto anti-CD3 antibody-coated plates and co-cultured with either WT or OGR1-KO macrophages (mac) that had been pre-stimulated with 0.1 μg/mL LPS. The percentage of divided CD4+ cells (A-B) was examined after a 3-day incubation period. (A) shows representative data while (B) shows means + SEM of values obtained from 6 individual experiments. *p< 0.05 by Student’s t-test (two-tailed). (C-D) WT CD4+ T cells were co-cultured with either WT or OGR1-KO macrophages, at a ratio of 1:0.2, for 5 days (C) or 4 days (D) and supernatants were used to measure the levels of IFN-γor IL-17 by ELISA. Data are means + SEM of triplicate cultures and are representative of 3 independent experiments. *p<0.05, **p<0.01 Student’s t-test (two-tailed).