Table 1. Concentration and fold change of 1C metabolites in parental MEFs and their iPS cell derivatives.
| Metabolite | [Metabolite]a MEFs | [Metabolite]a iPS cells | Fold-change [iPS]/[MEFs] |
|---|---|---|---|
| 5-Adenosyl-methionine (SAM) | 0.30 ± 0.04 | 4.2 ± 1.1 | 13.8 |
| NADPH | 0.05 ± 0.03 | 0.43 ± 0.06 | 7.6 |
| Choline | 2454 ± 109 | 15391 ± 600 | 6.3 |
| 5-methyl-tetrahydrofolate (5-mTHF) | 0.006. ± 0.001 | 0.021 ± 0.008 | 3.5 |
| ATP | 2.5 ± 0.2 | 7.3 ± 1.6 | 2.8 |
| 5-Adenosyl-homocysteine (SAH) | 0.6 ± 0.1 | 1.0 ± 0.1 | 1.6 |
| Folic acid | 0.19 ± 0.05 | 0.27 ± 0.02 | 1.5 |
| dUMP | N.D. | 8.2 ± 2.7 | >> |
| Cystathionine | 0.3 ± 0.1 | 0.38 ± 0.03 | 1.25 |
| Serine | 37 ± 5 | 36.3 ± 1.4 | 1.0 |
| Thymidine 5-phosphate (dTMP) | 0.64 ± 0.09 | 0.61 ± 0.09 | 1.0 |
| Dimethylglycine | 108 ± 5 | 102 ± 16 | 0.9 |
| Methionine | 2.5 ± 0.5 | 2.2 ± 0.5 | 0.9 |
| Glycine | 42 ± 19 | 23 ± 5 | 0.6 |
| Pyridoxal 5-phosphate (B6) | 2.2 ± 0.5 | 0.86 ± 0.05 | 0.4 |
| Betaine | 1.9 ± 0.1 | 0.52 ± 0.02 | 0.3 |
| Homocysteine (HCy) | 0.49 ± 0.04 | 0.13 ± 0.02 | 0.3 |
| Riboflavin (B2) | 0.037 ± 0.001 | 0.006 ± 0.002 | 0.2 |
| Taurine | 24 ± 3 | 3.7 ± 0.7 | 0.15 |
| Cysteine | 0.32 ± 0.02 | 0.052 ± 0.008 | 0.1 |
| Dihydrofolate (DHF) | 0.039 ± 0.01 | 0.008 ± 0.003 | 0.06 |
| Cyanocobalamin (B12) | N.D. | N.D. | - |
| Folinic acid (5-formyl-tetrahydrofolate) | N.D. | N.D. | - |
| Methylcobalamine | N.D. | N.D. | - |
| Tetrahydrofolate (THF) | N.D. | N.D. | - |
a
[Metabolite] in mean (μM/mg of protein) ± SD. N.D. Not detectable. Decimals were set according to the first significant digit of the measure SD.