Figure 1. Huaier extract inhibited the proliferation of 4T1 cells and infiltration of macrophages.
(A) Huaier extract inhibited 4T1 cells growth in vivo after administrated with Huaier extract for 21 days. (B) The tumor growth curves. (C) Representative IHC images of CD206 positive macrophages in tumor tissues. (D) 24 h migration assay of RAW264.7 cells treated with 0 mg/ml, 4 mg/ml, 8 mg/ml Huaier extract. (E) 24 h migration assay of RAW264.7 cells to CM from 0 mg/ml, 4 mg/ml and 8 mg/ml treated 4T1 cells. (F) The tumor weight in control group and Huaier group. (G) The number of M2 positive macrophages infiltration in control group and Huaier group. (H) The number of macrophages migrated in Figure C. (I) The number of macrophages migrated in Figure D. (J) The relative mRNA expression of CSF-1, GM-CSF and VEGF. Results are presented as the mean ± SEM of triplicate samples. *P < 0.05, **P < 0.01.