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. 2016 Feb 1;6:20049. doi: 10.1038/srep20049

Figure 3. Huaier extract inhibited the macrophages induced angiogenesis.

Figure 3

(A) Representative IHC images of CD31 in tumor tissues in Huaier group and control group. (B) Micro vessels density in control group and Huaier group. (C) HUVEC migration response to CM from 0 mg/ml, 4 mg/ml, 8 mg/ml Huaier extract treated RAW264.7 cells for 24 h. (D) Tube formation assay of HUVEC cells treated with DMEM + 5%FBS (Negative control) or CM + 5%FBS for 4 h. (E) CAM assay treated with CM from 0 mg/ml, 4 mg/ml, 8 mg/ml Huaier extract treated RAW264.7 cells for 72 h. (F) Number of complete tube-like structures. Results are presented as the mean ± SEM of triplicate samples. *P < 0.05, **P < 0.01.