Figure 2. Enhancement of Yorkie-induced eye overgrowth by VDRC KK RNAi lines co-segregates with insertion at 40D and is independent of knockdown of intended target genes.

(a–j) Adult eye phenotypes of F₁ flies carrying GMR-Gal4, UAS-yki^S168A–YFP transgenes crossed to VDRC genetic background (a), VDRC line 107151 (MBD-like) with double insertion (b), single insertion at 30B (c) and 40D (d), VDRC line 105838 (Caf1) with double insertion (e), single insertion at 30B (f) and 40D (g), VDRC line 110512 (CG3630) with double insertion (h), single insertion at 30B (i) and 40D. The transgene insertion at 40D in line 110512 does not carry a functional hairpin-coding sequence, hence it is called ‘40D^UAS' throughout this manuscript. (j). (k,l) Eye phenotypes of flies harbouring sav³ clones expressing Gal4 in the eye combined with the VDRC genetic background (k) and 40D^UAS (l). Sav is an upstream negative regulator of Yki, therefore the sav³ mutation causes Yki hyperactivation. Clones were generated using the Mosaic analysis with a repressible cell marker (MARCM) system under the control of the eyeless (ey) promoter.