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. 2014 Dec 12;7(3):109–121. doi: 10.3727/215517914X681794

Figure 4.

Figure 4

Mucin 5AC expression. Representative images of mucin 5AC expression (red) for each condition at 7, 14, and 21 days of culture (A–F). Both experimental conditions showed an increase in expression over time, but the rate was significantly higher for the rotating ALI cultures, as can be seen by comparing (C) and (F). Nuclei are stained with DAPI (blue). Quantitative measure of mucin expression over time for both experimental conditions, expressed as percentage of cells staining positive, with 95% confidence intervals (G). RT-PCR analysis of mucin 5AC expression in NHBE cells cultured in rotating ALI system at day 14 and day 21 compared to the cells cultured in static conditions (H).