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. 2016 Jan 21;18(1):10–24. doi: 10.1016/j.neo.2015.11.010

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© 2015 Institut National de la Santé Et de la Recherche Médicale

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Supplementary Figure S6. — (A) Staining of AMOTL1 expression in cryosections of BC52 tet on AL1 cell aggregates in Matrigel, with (DOX +) or without (DOX −) doxycycline induction. (B) The expression of cleaved caspase 3 was evaluated by immunofluorescence staining performed on aggregate cryosections of BC52 tet on AL1 cell aggregates in Matrigel, with (DOX +) or without (DOX −) doxycycline induction. The percentage of cleaved caspase-3 positive is presented. No significant difference was observed (Mann-Whitney test). (C) The expression of cleaved caspase-3 was evaluated by IHC staining performed on tumor sections of mice fed with (DOX +) or without (DOX −) doxycycline in the drinking water. The number of positive caspase-3 cells per high-power field is presented on the left. The right panel shows immunoblot analysis of cleaved caspase 3 levels in tumor samples of mice fed with (DOX +) or without (DOX −) doxycycline. No significant difference was observed (Mann-Whitney test).