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. 2016 Feb 2;7:64. doi: 10.3389/fmicb.2016.00064

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Copyright © 2016 Gil-Bona, Reales-Calderon, Parra-Giraldo, Martinez-Lopez, Monteoliva and Gil.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ecm33 is important for nuclear DNA division, septa distribution and actin polarization during C. albicans growth. (A) calcofluor staining was used to visualize the chitin localized in the septum. (B) The nuclear DNA was observed by labeling with DAPI, a highly specific stain for DNA. Arrows indicate defects in the number of nuclei. (C) C. albicans strains were fixed and stained with Alexa phalloidin to visualize actin. Arrows in SC5314 indicate normal actin polarization in buds, compared to RML2U.