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Ecm33 possitively influences the chronological life span of C. albicans. (A) SC5314 and RML2U were logarithmically growth in SD medium at 30°C. Samples were taken at the indicated times to determine CLS, as described in Section “Materials and Methods.” (B) CLS under calorie restriction by glucose reduction (0.5% glucose SD). (C) Extreme calorie restriction in which cultures were transferred to water on day 3 further extends the life span of RML2U.
