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. 2016 Feb 2;7:64. doi: 10.3389/fmicb.2016.00064

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Copyright © 2016 Gil-Bona, Reales-Calderon, Parra-Giraldo, Martinez-Lopez, Monteoliva and Gil.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Measurement of the apoptotic status of C. albicans strains after the interaction with murine macrophages. (A) Percentage of C. albicans cells that contain ROS after 3, 6, and 8 h of macrophage interaction. (B) Representative fluorescence images of C. albicans culture stained with DHR 123. (C) Percentage of C. albicans cells with active caspase revealed by the caspase inhibitor VAD-FMK-FITC. (D) Representative fluorescence micrographs showing caspase activity in C. albicans cells. Statistically significant differences relative to the RML2U control are indicated (^∗p < 0.05).