Skip to main content
. 2016 Feb 2;6:19799. doi: 10.1038/srep19799

Figure 1. Expression, purification and characterization of two novel CTX derivatives.

Figure 1

(A) Multiple sequence alignments of Chlorotoxin (CTX), BmKCT, and the two derivatives CA4 and CTX-23. The conserved amino acid residues are highlighted with black background, and the mutated amino acid residues are indicated in color. (B) HPLC purification and SDS-PAGE analysis of the derivative peptide CA4. The expressed and purified GST-CA4 fusion protein was digested with enterokinase, after which RP-HPLC was used to separate GST protein and CA4 peptide, corresponding to peaks at elution time from 18 min to 18.5 min and from 27 min to 30 min, respectively. (C) HPLC purification and SDS-PAGE analysis of the derivative peptide CTX-23. The expressed and purified GST-CTX fusion protein was digested with enterokinase, after which RP-HPLC was used to separate GST protein and CTX-23 peptide, corresponding to peaks at elution time from 19.5 min to 20 min and from 27 min to 30 min, respectively.