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. 2016 Feb 2;6:19799. doi: 10.1038/srep19799

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Chlorotoxin (CTX) and its derivatives CA4 and CTX-23 were tested on rodent and human glioma cells and on primary human glioma cells clone TN22. (A) Cell viability assay of chlorotoxin and its analogues treated rodent gliomas (F98) for 48 hours. BSA served as a control for potential osmotic effects. (B) Cell viability assay of CTX and its analogues applied to human gliomas (U251) for 48 hours. BSA served as a control for potential osmotic effects. After the indicated time point, cell viability was determined. (C) CTX and CA4 treatment on primary human glioma cells for 48 hours. Quantification is given from three independent experiments. Means are given as ± SD, n = 8 per group, *P < 0.05 (Student’s unpaired t-test).