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. 2015 Jan 22;11(6):4573–4578. doi: 10.3892/mmr.2015.3238

Figure 5.

Figure 5

Effects of pre-treatment with TPA on TM-induced apoptosis in HL-60 cells. (A) HL-60 cells were seeded in 100 mm culture dishes and treated with 0.32 nM TPA for different time intervals. Activation of NF-κB in HL-60 cells was determined by electrophoretic mobility shift assay. P=probe only. C=control. Treatment of HL-60 cells with TPA for 1 h resulted in a marked activation of NF-κB. (B and C) HL-60 cells were seeded at a density of 1×105 cells/ml in 35 mm culture dishes. The cells were treated with TM for 48 h with or without pre-treatment with 0.32 nM TPA for 1 h. (B) The number of viable cells was determined by the trypan blue exclusion assay and expressed as a percentage of the control. (C) Apoptosis was determined by propidium iodide staining and morphological assessment. TPA, 12-O-tetradecanoylphorbol-13-acetate; TM, α-tomatine; CH, cholesterol; DMSO, dimethyl sulfoxide.