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. 2015 Jul 14;34(3):1589–1598. doi: 10.3892/or.2015.4131

Figure 5.

Figure 5

Analysis of poly(ADP-ribose)-mediated caspase-independent cell death in breast adenocarcinoma cells after TRPM2 inhibition and chemotherapeutic treatments. Immunoblot detection of (A) apoptosis-inducing factor (AIF) and (B) poly(ADP-ribose) glycohydrolase (PARG) in MDA-MB-231 breast adenocarcinoma cells after RNAi silencing. Loading controls for immunoblots were provided by the immunodetection of β-actin. Con, untransfected cells; Scr, cells transfected with negative control scrambled siRNA oligos. (C) Quantification of cell death by flow cytometry was performed in MDA-MB-231 cells after RNAi knockdown of AIF or PARG, pretreatment with 20 μM ACA for 30 min and treatment with 100 μM MNNG. *p<0.05, one-way ANOVA and unpaired Student's t-test; error bars represent the SEM.