Figure 4.

Gefitinib/SU11274 combination reduces survival of TNBC cells through inducing G2 arrest. (A) Cells were treated with compounds (2.5 μM for HS578T or 10 μM for MDA-MB-231) for 24 h and further cultivated for 10–14 days in normal growth media. The survived colonies were stained as described in Materials and methods. Left, representative images from two independent experiments performed in triplicate are shown. Right, relative amount of survived colonies were determined as described in Materials and methods. Gef, gefitinib, SU, SU11274; Combo, combination. ^***P<0.001. (B) Cells were treated with 10 μM compounds as indicated for 24 h and the cell cycle distribution was determined by FACS analysis. (C) Cells were treated as indicated for 24 h and western blot analysis was performed with indicated antibodies. β-actin was used as a loading control.