Figure 7.

Knockdown of RPS6 reduces the proliferation of TNBC cells. (A) HS578T and MDA-MB-231 cells were transfected with either control- and RPS6-siRNA as described in Materials and methods and the number of viable cells was determined by counting viable cells with AO/PI staining. Representative data are presented as mean ± SEM from two independent experiments performed in triplicate. ^**P<0.01; ^***P<0.001. (B) Cells were transfected with siRNAs as described (A) and western blot analysis was performed with indicated antibodies. β-actin was used as a loading control.