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. 2016 Feb 2;6:20193. doi: 10.1038/srep20193

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Copyright © 2016, Macmillan Publishers Limited

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(a) Timeline of the ages and estimated WT cell type distribution of seminiferous tubules of mice used for this study. The height of each designated region represents the estimated proportion of total number of cells in an average seminiferous tubule. (b,c) MESDA for SMN2 (b) and hybrid Smn (c); n = 2 mice per genotype. Isoform identities are given on the right side of each gel image where Δ indicates a skipped exon. Each lane is a representative example taken from two replicates. Lower panels show results of densitometric quantification of the most prominent isoforms of each gene. (d) Western blots for Tra2β, hnRNP Q, hnRNP A1, hnRNP A2/B1, and Actin during testis development. Equal amounts of protein from each of 3–4 C/C samples were pooled together and 15 μg of protein loaded for each time point. Densitometric quantifications are given on the right. Abbreviation: SZ, spermatozoa.