Skip to main content
. 2015 Dec 21;5(2):159–168. doi: 10.1002/cam4.572

Figure 2.

Figure 2

RT‐PCR assay for the NAB2‐STAT6 fusion transcripts of the intrathoracic solitary fibrous tumors (SFTs). (A) PCR products of representative cases with 5 different exon composition types were detected by three primer pairs labeled as I‐III. The lane loaded with a 100‐bp DNA ladder marker was labeled as M, followed by Arabic numbers 1–10 for individual SFTs from left to right. The asterisked samples were validated by Sanger sequencing. (B) Partial sequencing chromatograms showed junctions of the NAB2‐STAT6 chimeric transcripts in the representative cases. Note that the inserted NAB2 and STAT6 intronic sequences, as indicated by the thin overhead horizontal lines, were identified in the case with the NAB2ex4‐STAT6ex3 variant (lane 5) and one of 4 cases with NAB2ex6‐STAT6ex17 variant (lane 8), respectively. The downward arrows indicates nucleotides of undetermined origins between the exons and introns, including one cytosine behind the NAB2 intron 4 (8 bp) and one cytosine located 5′ to the STAT6 intron 16 (17 bp). #, exon integrity interrupted by the junction breakpoints; I, NAB2 or STAT6 intron.