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. 2015 Dec 13;5(2):315–324. doi: 10.1002/cam4.567

Figure 3.

Figure 3

miR‐548d‐3p downregulates the expression of TP53BP2 by directly targeting the mRNA 3′UTR. (A) The TP53BP2 3′UTR reporter in target validation of miR‐548d‐3p. Mutant UTR has a 6 bp replaced by miRNA seed sequence. (B) miR‐548d‐3p specifically represses its targets in the luciferase assay, while cannot influence the mutant UTR luciferase assay. *P < 0.05 versus the corresponding control. (C) mRNA level of TP53BP2 by qRT‐PCR in the MDA‐MB‐231 transfected by pre‐miR‐548d‐3p and control miRNA. **P < 0.01 versus the corresponding control. (D) Protein level of TP53BP2 by western blot.