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. 2016 Jan 20;7:10342. doi: 10.1038/ncomms10342

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EM images of areas of intercellular contact (a) were traced for easier visualization (b). Same sample was processed for detection of N-cadherin (purple; c) and Na_V1.5 (green; d) by SMLM. The EM and SMLM images were aligned by unbiased fiducial markers (see also Supplementary Fig. 1 and Methods), allowing identification of Na_V1.5 clusters localized to the intercellular membrane, and their proximity to N-cadherin (e). Scale bar, 1 μm.