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. 2016 Feb 2;6:20287. doi: 10.1038/srep20287

Figure 8. Evaluation of migratory capacity of UC-MSCs, UC-MSCs-vector and UC-MSCs-IGF-1.

Figure 8

(a) Migratory capacity of UC-MSCs, UC-MSCs-vector and UC-MSCs-IGF-1 in vivo. Representative images of hNA-positive cells in the UC-MSCs group, UC-MSCs-vector group and UC-MSCs-IGF-1 group. The number of BrdU-positive cells was further counted in each field. (b) Evaluation of migratory capacity of UC-MSCs, UC-MSCs-vector and UC-MSCs-IGF-1 in vitro. Expression of the genes about migratory capacity (FCER1G, ITGB2, C3AR1, DDR1, LRP1 and PDGFB) was detected with qPCR. The level of gene expression in UC-MSCs was regarded as 1.0. The cell migratory capacity was further evaluated using transfilter assay. The number of migrating cells was counted in each field. *P < 0.05 compared with the normal UC-MSCs group and UC-MSCs-vector group respectively. (c) Effect of IGF-1-siRNA on the migratory capacity of UC-MSCs-IGF-1. Expression of FCER1G, ITGB2, C3AR1, DDR1, LRP1 and PDGFB was further detected with qPCR in UC-MSCs-IGF-1, UC-MSCs-control, UC-MSCs-siRNA. The level of gene expression in UC-MSCs-IGF-1 was regarded as 1.0. The transfilter assay was also performed to determine the cell migratory capacity as before.